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Equine porphyrins. Co-oxidation of meso-tetraphenylporphyrin TPPH to form four different isomeric porphyrins has been undertaken. Small-molecular mass porphyrins in which a carboxylic acid group replaces the chlorine atom in TPPH have been prepared. The molecular masses of these compounds were determined by mass spectroscopy, and their structure was confirmed by single-crystal X-ray diffraction.
The porphyrins have been used to determine the excitation spectra of TPPH and its isomeric porphyrins. Spectral measurements were performed by flash photolysis, steady-state absorption, and steady-state fluorescence. The excitation spectra of all the porphyrins form a continuum, with the fluorescence maximized at approximately nm.
The fluorescence quantum yield of TPPH was determined to be approximately 0. The significance of these results is that they demonstrate that structural modification of the axial ligand of the porphyrin ring can shift both the emission and the absorption spectra.
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